云南乌金猪与约大乌猪低氧适应性的研究

doi:10.11843/j.issn.0366-6964.2015.06.012

Abstract

Abstract:

This study aimed to ascertain the differences of hypoxia adaptation between Yunnan plateau Wujin pig and Yuedawu pig.Blood samples of 30 Wujin pigs and 30 Yuedawu pigs were collected（Half male and female）on 60 days.The blood HGB，HCT，RBC，MCH，MCHC and oxygen saturation were determined.The gene expressions of HIF-1α ，VEGF and EPO were determined by real-time PCR in heart，liver，lung，kidney，jejunum，ileum，duodenum，skin，muscle，pancreas，testes and ovaries of Yuedawu and Wujin pigs（half male and female）.The results showed that HGB and RBC of Wujin pigs was significant higher than that of Yuedawu pigs(P<0.01)，and hematocrit (HCT) and MCH was lower than Yuedawu pigs.Oxygen saturation of Wujin pigs was higher than that of Yuedawu pigs，without significant differences(P>0.05).HIF-1α，VEGF， EPO mRNA were detected in various tissues of Yuedawu and Wujin pigs.The gene expressions of HIF-1α，VEGF and EPO in most tissues of Yuedawu pigs were higher than that in Wujin pigs，and main expression tissues for HIF-1α and VEGF were liver，lungs and pancreas in 2 pig breeds，whereas EPO was mainly expressed in kidney and liver，the trends of HIF-1α，VEGF and EPO genes expression in 2 pig breeds is consistent.In 2 pig breeds，the hypoxia adaptation gene expressions of sows were significantly higher than that of boar.The HGB，RBC and oxygen saturation of Wujin pigs was significantly higher than that of Yuedawu pigs.Physiological characterization parameters of hypoxia adaptation of Wujin pig were significantly higher than Yuedawu pig.Hypoxia adaptation gene expression in most tissues of Yuedawu pig were higher than that in Wujin pig.But the mechanisms of hypoxia adaptation need to be researched in the further.

CLC Number:

S828
S813.3

LI Mei-quan，ZHANG Chun-yong，AN Qing-cong，PAN Hong-bin，CHEN Ke-lin，GUO Rong-fu. The Study of Hypoxia Adaptive Differences of Yunnan Wujin and Yuedawu Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 965-973.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.06.012

https://www.xmsyxb.com/EN/Y2015/V46/I6/965

References

［1］SEMENZA G L，NEJFELLT M K，CHI S M，et al.Hypoxia-inducible nuclear factors bind to an enhancer element located 3′ to the human erythropoietin gene［J］.Proc Natl Acad Sci USA，1991，88：5680-5684.
［2］SEMENZA G L.Regulation of mammalian O₂ homeostasis by hypoxia-inducible factor 1［J］.Annu Rev Cell Dev Biol，1999，15：551-578.
［3］SEMENZA G L.HIF-1：mediator of physiological and pathophysiological responses to hypoxia［J］.Appl Physiol，2000，88(4)：1474-1480.
［4］JIANG B H，ZHENG J Z，LEUNG S W，et al.Transactivation and inhibitory domains of hypoxia-inducible factor 1［J］.J Biol Chem，1997，272：19253-19260.
［5］KALOUSI A，MYLONIS I，POLITOU A S，et al.Casein kinase 1 regulates human hypoxia-inducible factor HIF-1［J］.J Cell Sci，2010，123：2976-2986.
［6］SEMENZA G L.Hypoxia-inducible factor 1：master regulator of O₂ homeostasis［J］.Curr Opin Genet Dev，1998，8(5)：588-594.
［7］MYLONIS I，SEMBONGI H，BEFANI C，et al.Hypoxia causes triglyceride accumulation by HIF-1-mediated stimulation of lipin 1 expression［J］.J Cell Sci，2012，125：3485-3493.
［8］PARSANEJAD M，ZHANG Y，QU D，et al.Regulation of the VHL/HIF-1 pathway by DJ-1［J］.J Neurosci，2014，34：8043-8050.
［9］DE FRANCESCO E M，PELLEGRINO M，SANTOLLA M F，et al.GPER mediates activation of HIF-1α/VEGF signaling by estrogens［J］.Cancer Res，2014，74(15)：4053-4064.
［10］FERRARA N.Vascular endothelial growth factor［J］.Arterioscler Thromb Vasc Biol，2009，29(6)：789-791.
［11］祁玥，郑亚宁.血管内皮生长因子的研究进展［J］.青海师范大学学报(自然科学版)，2010，26(4)：61-66.
QI Y，ZHENG Y N.Research of the VEGF［J］.Journal of Qinghai Normal University (Natural Science Edition).2010，26(4)：61-66.(in Chinese)
［12］秦川，肖颖彬，陈林，等.促红细胞生成素预处理在大鼠心肌缺氧复氧损伤中的抗氧化效应［J］.局解手术学杂志，2011，20(1)：71-73.
QIN C，XIAO Y B，CHEN L，et al.Antioxidation effect of erythropoietin pretreatment on myocardium exposed to hypoxia-reoxygenation injury［J］.Journal of Regional Anatomy and Operative Surgery，2011，20(1)：71-73.(in Chinese)
［13］VESEY D A，CHEUNG C，PAT B，et al.Erythropoietin protects against ischaemic acute renal injury［J］.Nephrol Dial Transplant，2004，19(2)：348-355.
［14］JESSEN T H，WEBER R E，FERMIN G，et al.Adaptation of bird hemoglobins to high altitudes：demonstration of molecular mechanism by protein engineering［J］.PNAS，1991，88(15)：6519-6522.
［15］YOON D，PONKA P，PRCHAL J T，et al.Hypoxia and hematopoiesis［J］.Am J Physiol Cell Physiol，2011，300：C1215-C1222.
［16］刘金凤，商鹏，旦增旺久，等.藏猪促红细胞生成素（EPO）基因多态性分析［J］.湖南农业科学，2012，51(12)：2590-2592.(in Chinese)
LIU J F，SHANG P，DANZENG W J，et al.The polymorphism analysis of erythropoietin in Tibetan pig［J］.Hubei Agricultural Sciences，2012，51(12)：2590-2592.(in Chinese)
［17］PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR［J］.Nucleic Acids Res，2001，29(9)：e45.
［18］苟潇.藏鸡胚胎低氧适应的血红蛋白分子机制［D］.北京：中国农业大学，2005.
GOU X.Hemoglobin mechanism of hypoxia toleration in Tibetan chick embryo［D］.Beijing：China Agricultural University，2005.(in Chinese)
［19］强巴央宗，张浩，白玛央宗，等.高原环境中藏猪血液生理指标测定与比较［J］.西南农业学报，2011，24（6）：2382-2384.
CHAMBA Y Z，ZHANG H，BAINA Y Z，et al.Determination of blood physiological parameters in Tibet pig at high altitude［J］.Southwest China Journal of Agricultural Sciences，2011，24（6）：2382-2384.(in Chinese)
［20］赵同标，赵新全，常智杰，等.常氧下高原鼠兔HIF-lα mRNA的表达［J］.动物学研究，2004，25(2)：132-136.
ZHAO T B，ZHAO X Q，CHANG Z J，et al.Tissue specific expression of plateau pikas (Ochotona curzoniae) HIF-1α mRNA under normal oxygen［J］. Zoological Research，2004，25(2)：132-136.(in Chinese)
［21］TACCHINI L，DANSI P，MATTEUCCI E，et al.Hepatocyte growth factor signalling stimulates hypoxia inducible factor-1 (HIF-1) activity in HepG2 hepatoma cells［J］.Carcinogenesis，2001，22(9)：1363-1371.
［22］ANAVI S，HAHN OBERCYGER M，MADAR Z，et al.Mechanism for HIF-1 activation by cholesterol under normoxia：a redox signaling pathway for liver damage.［J］.Free Radic Biol Med，2014，71：61-69.
［23］LIANG Y，HUA Z，LIANG X，et al.The crystal structure of bar-headed goose hemoglobin in deoxy form：the allosteric mechanism of a hemoglobin species with high oxygen affinity［J］.J Mol Biol，2001，313(1)：123-137.
［24］ABBASI A，LUTFULLAH G.Molecular basis of bird respiration：primary hemoglobin structure component from Tufted duck (Aythya fuligula，Anseriformes)--role of alpha99Arg in formation of a complex salt bridge network［J］.Biochem Biophys Res Commun，2002，291(1)：176-184.
［25］邹多宏，黄远亮.低氧诱导因子-1α的研究进展［J］.国际口腔医学杂志，2010，37(3)：320-323.
ZOU D H，HUANG Y L.Research progress on hypoxia-inducible factor-lα［J］.International Journal of Stomatology，2010，37(3)：320-323.(in Chinese)
［26］KE X，FEI F，CHEN Y，et al.Hypoxia upregulates CD147 through a combined effect of HIF-1α and Sp1 to promote glycolysis and tumor progression in epithelial solid tumors［J］.Carcinogenesis，2012，33(8)：1598-1607.
［27］ZANJANI E D，POSTER J，BURLINGTON H，et al.Liver as the primary site of erythropoietin formation in the fetus［J］.J Lab Clin Med，1977，89(3)：640-644.
［28］PATEL N S，SHARPLES E J，CUZZOCREA S，et al.Pretreatment with EPO reduces the injury and dysfunction caused by ischemia/reperfusion in the mouse kidney in vivo［J］.Kidney Int，2004，66(3)：983-989.

[1]	WANG Ji-ying, WANG Yan-ping, XU Yun-hua, WANG Cheng, LIN Hai-chao, HU Hong-mei, WU Ying, GUO Jian-feng. Analyses of Intramuscular Fat Content, Fatty Acid Composition and the Related Traits in Lulai Black Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 585-594.
[2]	WANG Ping，HAO Wen-yan，CAO Li-hua，SHEN Kai-yuan，DAI Xiao-li，LI Hai-yan，LIANG Xian-wei，SHI De-shun，LI Xiang-ping. Cloning and Analysis of ssc-miR-148a Promoter [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1969-1976.
[3]	DING Rong-rong，YANG Ming，QUAN Jian-ping,YANG Lin-xue，LIU Jing-shun，LIU Jia，GU Ting，LIU De-wu，CAI Geng-yuan，WU Zhen-fang，YANG Jie. A Comparative Study of Carcass and Meat Quality Traits between DLY and PDLY Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1795-1805.
[4]	SUN Hao，WANG Zi-shuai，XU Yin-xue，TANG Zhong-lin. The Correlation between H19 Single Nucleotide Polymorphism and Pig Growth Characters and Its Spatio-temporal Expression [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 870-881.
[5]	HAN Li-qiang，GUO Yu-jie，LU Wei-fei，ZHANG Xin，CHU Bei-bei，WANG Jiang，YANG Guo-yu. Construction and Function Confirmation of MC4R Gene Eukaryotic Expression Vector with Different Mutations [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 679-685.
[6]	JI Jiu-xiu，FANG Shao-ming，ZHANG Zhen，ZENG Zhi-jun，MA Jun-wu，CHEN Cong-ying. Correlations of Blood Glucose and Lipids with Fat Deposition in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 686-692.
[7]	ZHANG Suo-yu，QIU Xiao-tian，DING Xiang-dong，ZHANG Qin，WANG Zhi-gang，WANG Chang-cun，QU Yun-long . Estimation of Genetic Parameters by Connecting Groups for Reproductive Traits of Yorkshire，Landrace and Duroc Pigs in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 429-438.
[8]	HONG Qian-long，ZHANG Ying，CAO Chun-wei，WANG Xian-long，ZHAO Jian-guo. Analysis of Sequence，Tissue Expression and Vector Construction of OCA2 Gene in Bama Miniature Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 439-448.
[9]	XU Pan，ZHANG Zhen，CUI Lei-lei，YANG Bin，DUAN Yan-yu. A Systems Genetics Study of Hematological Traits in a White Duroc × Erhualian Pigs F₂ Resource Population [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 232-240.
[10]	YANG Hui，FANG Shao-ming，HUANG Xiao-chang，CHEN Cong-ying，ZHANG Zhi-yan. Genome-Wide Association Study on Maternal Infanticide Behavior in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 241-248.
[11]	LI Man-man，WEN Bing-yan，ZHU He-shui，ZHONG Kai，YANG Guo-yu，WANG Yue-ying. Cloning of Porcine lipasin Gene and Its Effect on LPL Gene Transcription in Liver Cell [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 411-416.
[12]	ZHANG Dong-jie，LIU Di，ZHANG Xu，WANG Liang，YANG Guo-wei. Study of Pig MSTN Gene Point Mutation Based on the CRISPR-Cas9 System [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 207-212.
[13]	WANG Chen，QIN Ke，XUE Ming，MO De-lin，CHEN Yao-sheng，LIU Xiao-hong. Application of Genomic Selection in Swine Breeding [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 1-9.
[14]	WANG Shou-dong，FANG Guo-feng，ZENG Yong-qing，CHEN Wei，LI Chuan-hao，WANG Yan-dong. Preparation and Study of CuZnSOD Gene Transgenic Pigs in F₁ Generation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 16-24.
[15]	CHEN Chen，HU Xiong-gui，ZHU Ji，REN Hui-bo，DENG Yuan，PENG Ying-lin. Progress on the Research of miRNAs Associated with Fat Development in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2117-2126.

The Study of Hypoxia Adaptive Differences of Yunnan Wujin and Yuedawu Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments